Active Recombinant Mouse VEGF120 Protein, His & Avi-tagged, Biotinylated

• Cat.No.: VEGFA-377M
• Product Overview: Recombinant Mouse VEGF120 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 146 (Accession # Q00731-3). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag. The protein has a calculated MW of 17.3 kDa. The protein migrates as 22-24 kDa and 24-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His & Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized VEGFR1/R2 at 2 μg/mL (100 μL/well) can bind Recombinant Mouse VEGF120 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.06-2 ng/mL (QC tested).
  Immobilized Avastin at 5 μg/mL (100 μL/well) can bind Recombinant Mouse VEGF120 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.125-2 μg/mL (Routinely tested).
• Molecular Mass: 17.3 kDa
• Protein length: Ala 27 - Arg 146
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Mus musculus ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular permeability factor; Vpf; Vegf; Vegf120; Vegf164; Vegf188
• Gene ID: 22339
• mRNA Refseq: NM_001025250
• Protein Refseq: NP_001020421