|Product Overview :||Recombinant Mouse VEGF120 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 146 (Accession # Q00731-3). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag. The protein has a calculated MW of 17.3 kDa. The protein migrates as 22-24 kDa and 24-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.|
|Tag :||His & Avi|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity :||Immobilized VEGFR1/R2 at 2 μg/mL (100 μL/well) can bind Recombinant Mouse VEGF120 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.06-2 ng/mL (QC tested).
Immobilized Avastin at 5 μg/mL (100 μL/well) can bind Recombinant Mouse VEGF120 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.125-2 μg/mL (Routinely tested).
|Molecular Mass :||17.3 kDa|
|Protein length :||Ala 27 - Arg 146|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Notes :||Please avoid repeated freeze-thaw cycles.|
|Stability :||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name :||Vegfa vascular endothelial growth factor A [ Mus musculus ]|
|Official Symbol :||VEGFA|
|Synonyms :||VEGFA; vascular endothelial growth factor A; vascular permeability factor; Vpf; Vegf; Vegf120; Vegf164; Vegf188;|
|Gene ID :||22339|
|mRNA Refseq :||NM_001025250|
|Protein Refseq :||NP_001020421|