Recombinant Mouse Vegfa Protein, His & Avi-tagged, Biotinylated

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Recombinant Mouse Vegfa Protein, His & Avi-tagged, Biotinylated

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Cat.No. : Vegfa-729M
Product Overview : Biotinylated Recombinant Mouse Vegfa Protein (Ala 27 - Arg 190) (Q00731-2), fused with a polyhistidine tag at the N-terminus, followed by an Avi tag, was expressed in HEK293.
Source : HEK293
Species : Mouse
Tag : His & Avi
Predicted N Terminal : Ala 27
Form : Lyophilized from sterile PBS, pH7.4, 10% trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized VEGFR2/R3, Fc tag at 2μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF164, His Tag with a linear range of 0.019-0.51 ng/mL.
Molecular Mass : Recombinant Mouse Vegfa Protein has a calculated MW of 22.5 kDa. As a result of different glycosylation, The protein migrates as 28-33 kDa under reducing (R) condition and 46-60 kDa under non-reducing (NR) condition on SDS-PAGE gel.
Protein length : Ala 27 - Arg 190
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : Vegfa vascular endothelial growth factor A [ Mus musculus ]
Official Symbol : Vegfa
Synonyms : VEGFA; vascular endothelial growth factor A; vascular permeability factor; Vpf; Vegf; Vegf120; Vegf164; Vegf188;
Gene ID : 22339
mRNA Refseq : NM_001025250
Protein Refseq : NP_001020421
Pathway : Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem;
Function : cell surface binding; chemoattractant activity; cytokine activity; fibronectin binding; growth factor activity; heparin binding; platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity; receptor agonist activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor binding;

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