Recombinant Mouse Vps4b Protein, Myc/DDK-tagged

• Cat.No.: Vps4b-6944M
• Product Overview: Purified recombinant protein of Mouse vacuolar protein sorting 4B (Vps4b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 49.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Vps4b vacuolar protein sorting 4B [ Mus musculus (house mouse) ]
• Official Symbol: Vps4b
• Synonyms: VPS4B; vacuolar protein sorting 4b (yeast); vacuolar protein sorting-associated protein 4B; suppressor of K+ transport defect 1; suppressor of K(+) transport growth defect 1; Skd1; 8030489C12Rik
• Gene ID: 20479
• mRNA Refseq: NM_009190
• Protein Refseq: NP_033216
• UniProt ID: P46467