Recombinant Mouse Vps53 Protein, Myc/DDK-tagged

• Cat.No.: Vps53-6945M
• Product Overview: Purified recombinant protein of Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 73.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Vps53 VPS53 GARP complex subunit [ Mus musculus (house mouse) ]
• Official Symbol: Vps53
• Synonyms: Vps53; VPS53 GARP complex subunit; Hccs1; 2010002A08Rik; 2310040I21Rik; 3100002B05Rik; vacuolar protein sorting-associated protein 53 homolog
• Gene ID: 68299
• mRNA Refseq: NM_026664
• Protein Refseq: NP_080940
• UniProt ID: Q8CCB4