Recombinant Mouse Wif1 Protein, His-tagged

• Cat.No.: Wif1-372M
• Product Overview: Recombinant mouse WIF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: WIF1, also known as wnt inhibitory factor 1, is a secreted protein that binds to Wnt proteins and inhibits their activities. This protein signaling plays a pivotal role in skeletal development and in the control of cartilage and bone turnover. Also, WIF1 is present in fish, amphibia and mammals, and is expressed during Xenopus and zebrafish development in a complex pattern that includes paraxial presomitic mesoderm, notochord, branchial arches and neural crest derivatives.
• Source: Insect cell
• Species: Mouse
• Tag: His
• Form: Liquid. In 20mM MES (pH5.5) containing 1mM DTT, 1mM PMSF, 30% glycerol.
• Molecular Mass: 39.4kDa (359aa)40-57KDa (SDS-PAGE under reducing conditions.)
• AA Sequence: GQPPEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMP AIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVA AFEVNVIVMN SEGNTILRTP QNAIFFKTCQ QAECPGGCRN GGFCNERRVC ECPDGFYGPHCEKALCIPRC MNGGLCVTPG FCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCELSKC PQPCRNGGKC IGKSKCKCPK GYQGDLCSKP VCEPGCGAHG TCHEPNKCQC REGWHGRHCN KRYGASLMHA PRPAGAGLER HTPSLKKAED RRDPPESNYI WVEHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Wif1 Wnt inhibitory factor 1 [ Mus musculus ]
• Official Symbol: Wif1
• Synonyms: WIF1; Wnt inhibitory factor 1; wnt inhibitory factor 1; WIF-1; AW107799
• Gene ID: 24117
• mRNA Refseq: NM_011915
• Protein Refseq: NP_036045
• Pathway: Wnt signaling pathway, organism-specific biosystem; Wnt signaling pathway, conserved biosystem
• Function: Wnt-protein binding