Recombinant Ovine IL10 Protein

• Cat.No.: IL10-33O
• Product Overview: The Ovine IL-10 recombinant protein was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.

Specification

• Description: The IL-10 family of cytokines consists of nine members: IL-10, IL-19, IL-20, IL-22, IL-24, IL-26, IL-28A, IL-28B, and IL-29. These cytokines elicit diverse host defense mechanisms. IL-10 family cytokines are essential for maintaining the integrity and homeostasis of tissue epithelial layers. By promoting innate immune response, members of this family can limit the damage caused by viral and bacterial infections. They can also facilitate the tissue-healing process in injuries caused by infection or inflammation. IL-10 itself is an anti-inflammatory cytokine. IL-10 family cytokines have indispensable functions in many infectious and inflammatory diseases.
• Source: Yeast
• Species: Ovine
• Molecular Mass: 18.4 kDa
• AA Sequence: SRDASTLSDSSCTHFPASLPHMLRE LRAAFGKVKTFFQMKDQLNSMLLTQ SLLDDFKGYLGCQALSEMIQFYLEE VMPQAENHGPDIKEHVNSLGEKLKT LRLRLRRCHRFLPCENKSKAVEQVK RVFNMLQERGVYKAMSEFDIFINYI ESYMTTKM(158)
• Applications: The Ovine IL-10 endotoxin-free recombinant protein can be used in cell culture, as an IL-10 ELISA Standard, and as a Western Blot Control.

Gene Information

• Gene Name: IL10 interleukin 10 [ Capra hircus (goat) ]
• Official Symbol: IL10
• Synonyms: IL10; interleukin 10; IL-10; interleukin-10
• Gene ID: 100860746
• mRNA Refseq: XM_005690416
• Protein Refseq: XP_005690473
• UniProt ID: A0A452EY26

Reviews

09/27/2022

Effective in anti-inflammatory assays.

01/09/2019

High purity levels.

04/15/2017

Stable under long-term storage.

Q&As

What state-of-the-art experimental strategies, including advanced imaging techniques, are implemented to meticulously assess the in vivo efficacy of IL-10-based therapies across a spectrum of inflammatory disorders?

Cutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.

How are advanced methods like single-cell RNA sequencing and flow cytometry harnessed to investigate the immunomodulatory impact of IL-10 across distinct immune cell subtypes?

Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.

Could you elaborate on the intricate experimental methodologies applied to unravel the influence of genetic polymorphisms within IL-10 and its receptor on disease susceptibility?

Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.

How do researchers leverage advanced immunomonitoring tools to comprehensively evaluate the safety profiles of IL-10-based interventions in various clinical trial phases?

Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.

How do researchers employ in vitro models to elucidate the mechanisms underlying IL-10's anti-inflammatory effects on immune cells?

In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.

What sophisticated experimental techniques are utilized to dissect the intricate signaling pathways downstream of IL-10 activation?

State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.

Could you explain the multifaceted experimental strategies employed to determine the optimal dosage and administration routes for IL-10 in diverse animal models?

Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.

What innovative experimental designs are employed to explore potential synergies between IL-10 and other cytokines, unraveling complex interplay in immune regulation?

Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.

Can you elaborate on the comprehensive approaches scientists use to assess the tissue-specific expression patterns of IL-10 receptors in both normal and pathological conditions?

Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.

How are cutting-edge techniques such as CRISPR/Cas9 gene editing utilized to dissect the regulatory roles of IL-10 in complex autoimmune disease models?

CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.