Recombinant Ovine PRL Protein PRL-22O

Recombinant Ovine PRL Protein

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Recombinant Ovine PRL Protein

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Cat.No. : PRL-22O
Product Overview : Recombinant Prolactin Ovine produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids.
Description : Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.
Source : E. Coli
Species : Ovine
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Molecular Mass : 23 kDa
N-terminal Sequence Analysis : The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.
Residual Host Cell Protein Content_ : Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.
Purity : Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Storage : Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution Prl should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Concentration : 1 mg/mL
Storage Buffer : 0.0045 mM NaHCO3
Reconstitution : It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Gene Name : PRL
Official Symbol : PRL prolactin [ Ovis aries (sheep) ]
Synonyms : PRL; prolactin
Gene ID : 443317
mRNA Refseq : NM_001009306
Protein Refseq : NP_001009306

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