|Product Overview:||Recombinant Prolactin Ovine produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids.|
|Description:||Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.|
|Form:||Sterile Filtered White lyophilized (freeze-dried) powder.|
|Bio-activity:||Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.|
|Molecular Mass:||23 kDa|
|N-terminal Sequence Analysis:||The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.|
|Residual Host Cell Protein Content_:||Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.
|Purity:||Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
|Storage:||Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution Prl should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
|Storage Buffer:||0.0045 mM NaHCO3|
|Reconstitution:||It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.|
|Official Symbol:||PRL prolactin [ Ovis aries (sheep) ]|