Active Recombinant Ovine PRL Protein

• Cat.No.: PRL-22O
• Product Overview: Recombinant Prolactin Ovine produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids.

Specification

• Description: Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.
• Source: E. Coli
• Species: Ovine
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
• Molecular Mass: 23 kDa
• N-terminal Sequence Analysis: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.
• Residual Host Cell Protein Content_: Protein quantitation was carried out by two independent methods:
  1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
  2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.
• Purity: Greater than 98.0% as determined by:
  (a) Analysis by SEC-HPLC.
  (b) Analysis by SDS-PAGE.
• Storage: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution Prl should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  Please prevent freeze-thaw cycles.
• Concentration: 1 mg/mL
• Storage Buffer: 0.0045 mM NaHCO3
• Reconstitution: It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: PRL
• Official Symbol: PRL prolactin [ Ovis aries (sheep) ]
• Synonyms: PRL; prolactin
• Gene ID: 443317
• mRNA Refseq: NM_001009306
• Protein Refseq: NP_001009306