Recombinant Pan-species (General) MBP Protein, His/MBP-tagged

• Cat.No.: MBP-2755P
• Product Overview: Recombinant Pan-species (General) MBP Protein (Lys27-Thr392) with a N-His/MBP tag was expressed in E. coli.

Specification

• Description: Maltose-binding protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure.
• Source: E. coli
• Species: Pan-species (General)
• Tag: His&MBP
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 50 kDa
  Accurate Molecular Mass: 51 kDa
• Protein length: Lys27-Thr392
• Purity: > 95%
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Official Symbol: MBP
• Synonyms: Maltose Binding Protein; MBP

Gene Information

• Official Symbol: MBP
• Synonyms: Maltose Binding Protein; MBP

Reviews

12/21/2019

Product exactly as described.

07/27/2018

Worked well in ELISA.

05/13/2018

Excellent customer support, helpful.

Q&As

What is the primary function of the MBP gene and its encoded protein?

The MBP gene encodes the Myelin Basic Protein, which is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system.

How is MBP expression regulated during neural development?

MBP expression is tightly regulated during neural development. It increases as oligodendrocytes differentiate and begin to form the myelin sheath around axons.

What role does MBP play in multiple sclerosis (MS)?

In multiple sclerosis (MS), an autoimmune disease, MBP is one of the targets for immune cells. The immune system attacks and degrades the myelin sheath, leading to the characteristic symptoms of MS.

What are the implications of MBP in demyelinating diseases beyond MS?

Other than MS, demyelinating diseases like Pelizaeus-Merzbacher disease and some leukodystrophies can be linked to issues with MBP. These conditions can lead to a range of neurological symptoms due to impaired nerve transmission.

Are there any therapeutic strategies targeting MBP or its pathways in neurodegenerative diseases?

Therapeutic strategies targeting MBP mainly focus on preventing the immune system from attacking it, especially in the context of MS. This includes the development of immunomodulatory drugs and therapies to promote remyelination.

Are there any known diseases or conditions associated with mutations or dysregulation of MBP?

Beyond MS, mutations or dysregulation of MBP can lead to other rare demyelinating diseases and some forms of leukodystrophy.

How does MBP contribute to the structure and function of myelin sheaths in the nervous system?

MBP plays a crucial role in the formation of the myelin sheath, a fatty layer that insulates nerve fibers. It helps compact and stabilize the layers of myelin, ensuring efficient nerve signal transmission.