Active Recombinant Pig TF protein(Met1-Thr715), hFc-tagged

• Cat.No.: TF-568P
• Product Overview: Recombinant pig transferrin (NP_001231582.1) (Met 1-Thr 715) was expressed in HEK293, fused with the Human IgG1 Fc region at the C-terminus.

Specification

• Source: HEK293
• Species: Pig
• Tag: C-hFc
• Protein length: Met1-Thr715
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human His-TFRC functional ELISA. 2. Measured in a serum-free cell prolifeRation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.01-0.04 μg/mL.
• Molecular Mass: The secreted recombinant pig (Sus scrofa) transferrin/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 935 amino acids and has a predicted molecular mass of 103 kDa. It migRates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TF transferrin [ Sus scrofa ]
• Official Symbol: TF
• Synonyms: TF; transferrin; serotransferrin; siderophilin; beta-1 metal-binding globulin
• Gene ID: 396996
• mRNA Refseq: NM_001244653
• Protein Refseq: NP_001231582