Recombinant Plasmodium Falciparum AMA1 Protein (42-73aa & 227-271aa & 346-407aa & 452-488aa & 525-546aa), His-tagged

• Cat.No.: AMA1-2188P
• Product Overview: Recombinant Plasmodium Falciparum (isolate FC27/Papua New Guinea) AMA1 Protein (42-73aa & 227-271aa & 346-407aa & 452-488aa & 525-546aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.

Specification

• Description: Involved in parasite invasion of erythrocytes.
• Source: E. coli
• Species: Plasmodium Falciparum
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 27.5 kDa
• Protein length: 42-73aa & 227-271aa & 346-407aa & 452-488aa & 525-546aa
• AA Sequence: NEHREHPKEYQYPLHQEHTYQQEDS GEDENTLDNDKNSNYKYPAVYDDKD KKCHILYIAAQENNGPRYCNKDESK RNASDQPKQYEQHLTDYEKIKEGFK NKNASMIKSAFLPTGAFKADRYKSH GKGYNWGNYNTETQKEIERESKRIK LNDNDDEGNKKIIAPRIFISDDKDS LKEEYKDEYADIPEHKPTYDKMK
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.
• Synonyms: AMA-1; Merozoite surface antigen
• UniProt ID: P22621

Gene Information

• Synonyms: AMA-1; Merozoite surface antigen
• UniProt ID: P22621

Reviews

07/24/2022

Reduced assay time for accelerated data acquisition.

04/04/2020

Compatible with diverse sample preparation techniques.

02/19/2018

Reliable detection of protein-protein complex assembly.

Q&As

What are the antigenic properties of AMA1, and how can they be utilized for the development of diagnostic tools or vaccines against malaria?

AMA1 exhibits antigenic properties, and specific regions of the protein can be targeted for the development of diagnostic tests to detect malaria infection or for inclusion in vaccine candidates.

How does AMA1 interact with other invasion-related proteins, such as Rh family proteins or RON complex, and what is the functional significance of these interactions?

AMA1 interacts with other invasion-related proteins, such as Rh family proteins and the RON complex, forming a molecular machinery that coordinates the release of apical organelles and the establishment of the tight junction during invasion.

What are the downstream signaling events triggered by AMA1 binding, and how do they contribute to the establishment and maintenance of the Plasmodium infection?

AMA1 binding triggers signaling events that activate downstream pathways involved in parasite motility, cytoskeletal rearrangements, and formation of the parasitophorous vacuole, which are essential for successful invasion and establishment of the infection.

Can AMA1 be targeted by small molecules or antibodies to inhibit Plasmodium invasion, and what are the potential therapeutic implications of such interventions?

Inhibition of AMA1 function through small molecules or antibodies has shown promise as a potential therapeutic strategy to block Plasmodium invasion and prevent the establishment of malaria infection.

What are the evolutionary dynamics of AMA1, and how does it contribute to the adaptation and survival of Plasmodium species in different geographic regions?

AMA1 exhibits sequence diversity across different Plasmodium species, reflecting evolutionary pressures imposed by host immune responses and contributing to the adaptation of the parasite to different geographic regions and host populations.

What is the three-dimensional structure of the AMA1 protein, and how does it interact with its ligands or binding partners?

The three-dimensional structure of AMA1 reveals a conserved apical membrane antigen domain that interacts with its ligands, such as rhoptry neck proteins (RONs), to form a tight junction during invasion.

How does AMA1 trafficking occur within the parasite and its host cell, and what are the molecular mechanisms involved in its transport and localization?

AMA1 trafficking involves a complex network of parasite and host cell factors, including vesicular transport pathways, endosomal sorting machinery, and interactions with host cell membrane proteins.

How does AMA1 expression change during different stages of the Plasmodium life cycle, and what are the factors that regulate its expression?

AMA1 expression is upregulated during the asexual blood stage of the Plasmodium life cycle and is regulated by transcriptional and post-transcriptional mechanisms involving stage-specific transcription factors and RNA-binding proteins.

What are the genetic polymorphisms in AMA1, and how do they influence the parasite's ability to evade host immune responses or develop drug resistance?

Genetic polymorphisms in AMA1 have been associated with variations in antigenicity, immune evasion, and drug resistance in Plasmodium parasites, highlighting the importance of AMA1 as a target for surveillance and vaccine development.

What is the role of AMA1 in the invasion process of Plasmodium falciparum, and how does it contribute to the formation of the parasitic junction during erythrocyte invasion?

AMA1 plays a critical role in the invasion process of Plasmodium falciparum by facilitating the tight junction formation between the merozoite and the erythrocyte, enabling parasite entry into the host cell.