Recombinant Porcine CD163

• Cat.No.: CD163-94P
• Product Overview: CD163 Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 805 amino acids and having a molecular mass of 87kDa. The CD163 is fused to an 8 amino acid His Tag at C-terminus and purified by proprietary chromatographic techniques.

Specification

• Description: CD163 is an acute phase-regulated receptor which participates in the removal and endocytosis of hemoglobin/haptoglobin complexes by macrophages and thus keeps tissues from free hemoglobin-mediated oxidative damage. Furthermore, CD163 partakes in the uptake and recycling of iron, through endocytosis of hemoglobin/haptoglobin and ensuing breakdown of heme. In addition, CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent way. CD163 demonstrates greater affinity for complexes of hemoglobin and multimeric haptoglobin of HP-1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP-1S phenotype. Moreover, CD163 stimulates a cascade of intracellular signals which involves tyrosine kinase-dependent calcium recruitment, inositol triphosphate formation and secretion of IL-6 & CSF-1.
• Source: E. coli
• Species: Porcine
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4, containing 4M Urea.
• Molecular Mass: 87kDa
• AA Sequence: MDKLRMVLHE NSGSADLKLR VVDGVTECSG RLEVKFQGEW GTICDDGWDS DDAAVACKQL GCPTAVTAIG RVNASEGTGH IWLDSVSCHG HESALWQCRH HEWGKHYCNH NEDAGVTCSD GSDLELRLKG GGSHCAGTVE VEIQKLVGKV CDRSWGLKEA DVVCRQLGCG SALKTSYQVY SKTKATNTWL FVSSCNGNET SLWDCKNWQW GGLSCDHYDE AKITCSAHRK PRLVGGDIPC SGRVEVQHGD TWGTVCDSDF SLEAASVLCR ELQCGTVVSL LGGAHFGEGS GQIWAEEFQC EGHESHLSLC PVAPRPDGTC SHSRDVGVVC SRYTQIRLVN GKTPCEGRVE LNILGSWGSL CNSHWDMEDA HVLCQQLKCG VALSIPGGAP FGKGSEQVWR HMFHCTGTEK HMGDCSVTAL GASLCSSGQV ASVICSGNQS QTLSPCNSSS SDPSSSIISE ENGVACIGSG QLRLVDGGGR CAGRVEVYHE GSWGTICDDS WDLNDAHVVC KQLSCGWAIN ATGSAHFGEG TGPIWLDEIN CNGKESHIWQ CHSHGWGRHN CRHKEDAGVI CSEFMSLRLI SENSRETCAG RLEVFYNGAW GSVGKNSMSP ATVGVVCRQL GCADRGDISP ASSDKTVSRH MWVDNVQCPK GPDTLWQCPS SPWKKRLASP SEETWITCAN KIRLQEGNTN CSGRVEIWYG GSWGTVCDDS WDLEDAQVVC RQLGCGSALE AGKEAAFGQG TGPIWLNEVK CKGNETSLWD CPARSWGHSD CGHKEDAAVT CSEIAKSRES LHATGRSHHH HHHHH.
• Purity: Greater than 95.0% as determined by HPLC and SDS-PAGE.
• Stability: Lyophilized CD163 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD163 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized CD163 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Tag: Non
• Protein length: 805 a.a.

Gene Information

• Gene Name: CD163 CD163 molecule [ Sus scrofa (pig) ]
• Official Symbol: CD163
• Synonyms: CD163; CD163 molecule; scavenger receptor cysteine-rich type 1 protein M130; CD-163; M130; sCD163; MM130; Hemoglobin scavenger receptor; macrophage-associated antigen
• Gene ID: 397031
• mRNA Refseq: NM_213976
• Protein Refseq: NP_999141
• UniProt ID: Q2VL90
• Chromosome Location: Un
• Function: scavenger receptor activity; virus receptor activity

Reviews

01/25/2023

The outstanding performance of CD163 protein in both ELISA and protein electron microscopy structure analysis positions it as a highly valuable asset in diverse research areas.

09/09/2019

Its ability to accurately detect proteins and visualize their structures offers a valuable toolkit for advancing scientific understanding and discoveries.

01/13/2016

Whether it is protein-protein interactions, enzymatic assays, or cellular studies, CD163 protein delivers consistent and accurate data, making it a valuable tool for researchers.

Q&As

How does CD163 contribute to the understanding of cardiovascular diseases?

CD163 is implicated in the development and progression of cardiovascular diseases, serving as a marker for inflammation in atherosclerosis.

In what ways does CD163 contribute to the understanding of cancer progression?

CD163 expression is studied in the context of cancer to evaluate its role in promoting or inhibiting tumor growth and metastasis.

How is CD163 involved in the pathogenesis of neurodegenerative diseases?

CD163 is being investigated for its potential role in neurodegenerative diseases, where neuroinflammation is a key factor.

Are there diagnostic assays or imaging techniques that utilize CD163 as a molecular target?

Some diagnostic assays and imaging techniques use CD163 as a molecular target to visualize and quantify inflammatory processes in vivo.

How is CD163 linked to autoimmune disorders such as lupus?

CD163 expression is associated with the pathogenesis of autoimmune disorders, and its modulation is being explored for therapeutic purposes.