Recombinant Rabbit BPI Protein, His-tagged

• Cat.No.: BPI-327R
• Product Overview: Recombinant Rabbit BPI Protein (Ser315-Leu451) with N-His tag was expressed in E. coli.

Specification

• Description: The antimicrobial protein Bactericidal Permeability Increasing protein (BPI) is a 55 kDa protein found in the primary granules of polymorhonuclear leukocytes (PMN). The cytotoxicity action of BPI is limited to gram negative bacteria, reflecting the high affinity of BPI for bacterial LPS. Binding of BPI to live bacteria via LPS causes anti-infective activites: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils.
• Source: E. coli
• Species: Rabbit
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 18.4 kDa
  Accurate Molecular Mass: 18 kDa
• Protein length: Ser315-Leu451
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: BPI bactericidal/permeability-increasing protein [ Oryctolagus cuniculus (rabbit) ]
• Official Symbol: BPI
• Synonyms: BPI; bactericidal/permeability-increasing protein; bactericidal permeability-increasing protein
• Gene ID: 100009228
• mRNA Refseq: NM_001195804
• Protein Refseq: NP_001182733
• UniProt ID: G1T5Q9