Active Recombinant Rat Acvr2a protein, His-tagged, Low Endotoxin

• Cat.No.: ACVR2A-150R
• Product Overview: Recombinant Rat Acvr2a protein(P38444)(20-135 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 20-135 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Activin A, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Rat Activin RIIA, His Tag with the EC50 of 0.36μng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 15.06 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Acvr2a activin A receptor, type IIA [ Rattus norvegicus ]
• Official Symbol: Acvr2a
• Synonyms: ACVR2A; activin A receptor, type IIA; activin receptor type-2A; ACTR-IIA; activin receptor IIA; type II activin receptor; activin receptor type IIA; Acvr2; rActR-II
• Gene ID: 29263
• mRNA Refseq: NM_031571
• Protein Refseq: NP_113759