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Recombinant Rat Alox15b protein, His & T7-tagged

Cat.No. : Alox15b-6850R
Product Overview : Recombinant Rat Alox15b aa. (Asp349~Val633 (Accession # Q8K4F2)) fused with N-terminal His & T7 tag was produced in E. coli cells.
  • Specification
  • Gene Information
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Source : E. coli
Species : Rat
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 35.8kDa.
Protein length : Asp349~Val633 (Accession # Q8K4F2)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 5.6.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : Alox15b arachidonate 15-lipoxygenase, type B [ Rattus norvegicus (Norway rat) ]
Official Symbol : Alox15b
Synonyms : 15-LOX-2; 15-LOX-B; 15-lipoxygenase 2; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; linoleate 13-lipoxygenase 15-LOb; arachidonate 15-lipoxygenase B
Gene ID : 266604
mRNA Refseq : NM_153301.2
Protein Refseq : NP_695213.1
UniProt ID : Q8K4F2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (13)

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Does ALOX15B mutation cause autoimmune diseases? 04/18/2023

Yes, certain ALOX15B gene mutations are closely associated with the onset of several autoimmune diseases.

What is the relationship between the expression level of ALOX15B in tumors and patient prognosis? 11/25/2022

The expression level of ALOX15B in tumors is closely related to the treatment response and prognosis of patients.

What is the relationship between ALOX15B and diseases related to blood circulation system? 02/25/2022

Whether ALOX15B is related to the occurrence of diseases related to the blood circulation system (such as heart disease, thrombosis, etc.) still needs further research.

What diseases will be caused if the ALOX15B gene is mutated? 08/20/2021

Mutations in ALOX15B can lead to a variety of diseases, such as asthma and autoimmune diseases.

Does ALOX15B have application prospects in the treatment of skin diseases? 04/08/2021

Yes, ALOX15B may be a target for the treatment of certain skin diseases.

How does the expression level of ALOX15B in patients with asthma correlate with the severity of the disease? 01/24/2021

Studies have shown that the level of ALOX15B expression in asthma patients is positively correlated with the severity of the disease.

Does ALOX15B participate in the systemic inflammatory response of asthma? 10/27/2020

Yes, ALOX15B can promote an increased systemic inflammatory response in patients with asthma.

Does ALOX15B have potential application value in the treatment of tumors? 10/07/2020

Yes, some studies suggest that ALOX15B has the potential to be a new target for tumor therapy.

Is ALOX15B involved in tumor growth and development? 07/12/2020

Yes, ALOX15B is associated with the occurrence, development and metastasis of various cancers.

What is the role of ALOX15B in the human body? 04/25/2020

The enzyme encoded by ALOX15B is mainly involved in the metabolism of arachidonic acid (AA), which is closely related to biological processes such as inflammation and immune regulation.

What is the relationship between ALOX15B and asthma? 04/24/2020

Many experimental studies have shown that ALOX15B is closely related to the pathogenesis and pathophysiological mechanism of asthma.

What is the role of ALOX15B in angiogenesis? 03/22/2020

ALOX15B can inhibit angiogenesis and thus play a role in tumor growth and metastasis.

Is ALOX15B involved in the onset and progression of skin diseases? 06/30/2019

Yes, ALOX15B is closely related to the onset and progression of many skin diseases (such as psoriasis, psoriasis, etc.).

Customer Reviews (4)

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Reviews
05/22/2022

    By using this recombinant protein, the efficiency of my experiments was significantly improved, and unnecessary waste of time was also reduced.

    06/21/2021

      I appreciate the attention paid to product quality by this recombinant protein manufacturer, and their recombinant proteins are very stable, which makes our experiments smoother.

      03/06/2020

        The catalytic activity of this protein is very powerful and can effectively accelerate the process of chemical reactions, which brings great convenience to our experiments.

        09/22/2019

          The reproducibility of the Western blot was significantly improved using this recombinant protein.

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