Recombinant Rat AR Protein, His tagged

• Cat.No.: AR-743R
• Product Overview: Recombinant Rat AR Protein with His tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Description: Enables several functions, including androgen binding activity; androgen receptor binding activity; and nuclear receptor activity. Involved in several processes, including cellular response to hormone stimulus; mating; and regulation of transcription, DNA-templated. Located in several cellular components, including axon; dendrite; and nucleus. Used to study hypertension; osteoporosis; prostate adenocarcinoma; prostate cancer; and prostatic hypertrophy. Biomarker of acute kidney failure and androgen insensitivity syndrome. Human ortholog(s) of this gene implicated in several diseases, including attention deficit hyperactivity disorder; conduct disorder; disorder of sexual development (multiple); reproductive organ cancer (multiple); and spinal muscular atrophy (multiple). Orthologous to human AR (androgen receptor).
• Molecular Mass: The protein has a calculated MW of 61 kDa.
• AA Sequence: MHHHHHHLRMEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREAIQNPGPRHPEAASIAPPGACLQQRQETSPRRRRRQQHPEDGSPQAHIRGTTGYLALEEEQQPSQQQSASEGHPESGCLPEPGAATAPGKGLPQQPPAPPDQDDSAAPSTLSLLGPTFPGLSSCSADIKDILSEAGTMQLLQQQQQQQQQQQQQQQQQQQQQQEVISEGSSSVRAREATGAPSSSKDSYLGGNSTISDSAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYASLLGGPPAVRPTPCAPLAECKGLSLDEGPGKGTEETAEYSSFKGGYAKGLEGESLGCSGSSEAGSSGTLEIPSSLSLYKSGAVDEAAAYQNRDYYNFPLALSGPPHPPPPTHPHARIKLENPSDYGSAWAAAAAQCRYGDLASLHGGSVAGPSTGSPPATASSSWHTLFTAEEGQLYGPGGGGGSSSPSDAGPVAPYGYTRPPQGLASQEGDFSASEVWYPGGVVNRVPYPSPSCVKSEMGPWMENYSGPYGDMRLDSTRDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRA
• Purity: > 90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.5 mg/mL by BCA
• Storage Buffer: Sterile PBS, pH 7.4

Gene Information

• Gene Name: Ar androgen receptor [ Rattus norvegicus ]
• Official Symbol: Ar
• Synonyms: AR; androgen receptor; dihydrotestosterone receptor; nuclear receptor subfamily 3 group C member 4; androgen receptor (Testicular feminization), same as Tfm; Tfm; Andr
• Gene ID: 24208
• mRNA Refseq: NM_012502
• Protein Refseq: NP_036634
• UniProt ID: P15207

Citation

A detailed characterization of stepwise activation of the androgen receptor variant 7 in prostate cancer cells

Journal: Oncogene    PubMed ID: 33323969    Data: 2020/12/3

Authors: Carlos M. Roggero, Lianjin Jin, Yan Dong

Article Snippet:Fusion proteins were purified using glutathione-agarose beads (GE Life Sciences) or Ni-NTA resin (Thermo Fisher).Fusion proteins were purified using glutathione-agarose beads (GE Life Sciences) or Ni-NTA resin (Thermo Fisher).. For pull-down assays, 2 μg of His-AR-V7 or AR-FL recombinant protein (Creative BioMart) was incubated with 2 μg of GST-AR-V7 protein, GST alone, or GST-fused catalytic domain of JMJD2B that was immobilized on glutathione beads in 50 μl of binding buffer (25mM Tris, 0.1% TX-100, 0.5 M NaCl in water, and proteinase inhibitor cocktail) for 2 hr at 4°C on a rotating platform.. After centrifugation at 5000xg for 15 sec, the agarose beads were washed 4 times with the binding buffer.After centrifugation at 5000xg for 15 sec, the agarose beads were washed 4 times with the binding buffer.