Recombinant Rat Capn1 protein, His-tagged

• Cat.No.: Capn1-585R

Specification

• Product overview: Recombinant Rat Capn1 (P97571) aa. (Pro75~Asp356) fused with His tag was produced in E. coli cells.
• Description: Calcium-dependent protease that may be involved in experimental allergic encephalomyelitis (EAE).
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.5kDa; Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Pro75~Asp356
• Purity: >95%
• Characteristic: Isoelectric point is 5.3.
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Capn1 calpain 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Capn1
• Synonyms: Capn1; calpain 1; calpain-1 catalytic subunit; CANP 1; calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; calpain mu-type; calpain-1 large subunit; micromolar-calpain; muCANP
• Gene ID: 29153
• mRNA Refseq: NM_019152.2
• Protein Refseq: NP_062025.1
• UniProt ID: P97571