Recombinant Rat Cd274 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Cd274-561RAF555
• Product Overview: A DNA sequence encoding the rat CD274 (NP_001178883.1)(Met1-Thr238) (Alexa Fluor 555 conjugated) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The recombinant rat CD274/Fc is a disulfide-linked homodimer. The reduced monomer comprises 459 amino acids and has a predicted molecular mass of 51.8 kDa. The apparent molecular mass of the protein is approximately 73.2 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Thr238, 459
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: Cd274 CD274 molecule [ Rattus norvegicus ]
• Official Symbol: Cd274
• Synonyms: CD274; CD274 molecule; programmed cell death 1 ligand 1; Pdcd1lg1; RGD1566211
• Gene ID: 499342
• mRNA Refseq: NM_001191954
• Protein Refseq: NP_001178883