Recombinant Rat Cyp1b1 Protein


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Recombinant Rat Cyp1b1 Protein

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Cat.No. : Cyp1b1-140R
Product Overview : Recombinant rat Cyp1b1 protein and CYP-reductase without tag were coexpressed in E. coli.
Description : Plays a role in polycyclic aromatic hydrocarbon metabolism in adrenal microsomes.
Source : E. coli
Species : Rat
Bio-activity : Cytochrome c Reductase Activity: 1793 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: 7-ethoxyresorufin O-deethylase
Linear with time up to: 6 min(Determined at 0.1 µM substrate, using a P450 concentration of 1 pmol/ml)
Linear with P450 up to: 10 pmol/ml(Determined at 0.1 µM substrate, using an incubation time of 1 min)
Vmax: 2.1 pmol/min/pmol CYP
Km: 0.09 µM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 1.9 nmol/ml
Protein Concentration: 11.6 mg/ml
Specific P450 Content: 164 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : Cyp1b1 cytochrome P450, family 1, subfamily b, polypeptide 1 [ Rattus norvegicus (Norway rat) ]
Official Symbol : Cyp1b1
Synonyms : Cyp1b1; cytochrome P450, family 1, subfamily b, polypeptide 1; cytochrome P450 1B1; CYPIB1; P450RAP; cytochrome P450, subfamily 1B, polypeptide 1; cytochrome P450RAP; hydroperoxy icosatetraenoate dehydratase
Gene ID : 25426
mRNA Refseq : NM_012940
Protein Refseq : NP_037072
UniProt ID : Q64678

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