Active Recombinant Rat Cyp1b1 Protein

• Cat.No.: Cyp1b1-140R
• Product Overview: Recombinant rat Cyp1b1 protein and CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: Plays a role in polycyclic aromatic hydrocarbon metabolism in adrenal microsomes.
• Source: E. coli
• Species: Rat
• Bio-activity: Cytochrome c Reductase Activity: 1793 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: 7-ethoxyresorufin O-deethylase
  Linear with time up to: 6 min(Determined at 0.1 µM substrate, using a P450 concentration of 1 pmol/ml)
  Linear with P450 up to: 10 pmol/ml(Determined at 0.1 µM substrate, using an incubation time of 1 min)
  Vmax: 2.1 pmol/min/pmol CYP
  Km: 0.09 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.9 nmol/ml
  Protein Concentration: 11.6 mg/ml
  Specific P450 Content: 164 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: Cyp1b1 cytochrome P450, family 1, subfamily b, polypeptide 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Cyp1b1
• Synonyms: Cyp1b1; cytochrome P450, family 1, subfamily b, polypeptide 1; cytochrome P450 1B1; CYPIB1; P450RAP; cytochrome P450, subfamily 1B, polypeptide 1; cytochrome P450RAP; hydroperoxy icosatetraenoate dehydratase
• Gene ID: 25426
• mRNA Refseq: NM_012940
• Protein Refseq: NP_037072
• UniProt ID: Q64678