Active Recombinant Rat F3 protein, His-tagged

Cat.No. : F3-2735R
Product Overview : Active Recombinant Rat F3 protein(P42533)(30~252 aa), fused with N-terminal His tag, was expressed in E.coli.
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Species : Rat
Source : E.coli
Tag : His
Protein Length : 30-252 aa
Form : Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Bio-activity : TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was shown, and this effect was in a dose dependent manner.
Molecular Mass : 26.9kDa
Purity : > 95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name F3 coagulation factor III (thromboplastin, tissue factor) [ Rattus norvegicus ]
Official Symbol F3
Synonyms F3; coagulation factor III (thromboplastin, tissue factor); tissue factor; TF; coagulation factor 3; MGC93621;
Gene ID 25584
mRNA Refseq NM_013057
Protein Refseq NP_037189

Not For Human Consumption!

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