Active Recombinant Rat Fgf21 protein, His-tagged

• Cat.No.: Fgf21-931R
• Product Overview: Active Recombinant Rat Fgf21 protein(Q8VI80)(25-208 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 25-208 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Fibroblast growth factor 21 (FGF21) is a protein that in mammals is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family which possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF21 using Balb/c 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of FGF21 diluted with serum-free standard DMEM. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Balb/c 3T3 cells after incubation with FGF21 for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant FGF21 for 48h. It was obvious that FGF21 significantly increased cell viability of 3T3 cells. The ED50 of recombinant rat FGF21 is 0.23 ng/ml.
• Molecular Mass: 21.7kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Fgf21 fibroblast growth factor 21 [ Rattus norvegicus ]
• Official Symbol: Fgf21
• Synonyms: FGF21; fibroblast growth factor 21
• Gene ID: 170580
• mRNA Refseq: NM_130752
• Protein Refseq: NP_570108