Recombinant Rat FGFR4 protein(Met1-Asp367), hFc-tagged

• Cat.No.: FGFR4-1076R
• Product Overview: Recombinant Rat FGFR4 (Q498D6)(Met 1-Asp 367) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-hFc
• Protein length: Met1-Asp367
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4, The EC50 of Rat FGFR4 is 1.17 μg/mL. 3. Immobilized mouse FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4, The EC50 of Rat FGFR4 is 0.44 μg/mL. 4. Immobilized human bFGF at 10 μg/mL (100 μl/well) can bind Rat FGFR4, The EC50 of Rat FGFR4 is 0.163 μg/mL.
• Molecular Mass: The recombinant rat FGFR4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 592 amino acids and predicts a molecular mass of 66 kDa. The apparent molecular mass of the rat FGFR4/Fc monomer is approximately 110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fgfr4 fibroblast growth factor receptor 4 [ Rattus norvegicus ]
• Official Symbol: FGFR4
• Synonyms: FGFR4; fibroblast growth factor receptor 4; FGFR-4; MGC116290
• Gene ID: 25114
• mRNA Refseq: NM_001109904
• Protein Refseq: NP_001103374