Recombinant Rat Ghr protein(Met1-Arg265), His-tagged

• Cat.No.: Ghr-4090R
• Product Overview: Recombinant Rat GHR (P16310-1) (Met 1-Arg 265) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Protein length: Met1-Arg265
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit proliferation of INS-1 cells. The ED50 for this effect is 0.6-3 μg/mL in the presence of 50 ng/mL human growth hormone.
• Molecular Mass: The recombinant rat GHR comprises 258 amino acids and predicts a molecular mass of 29.6 kDa. The apparent molecular mass of the ratGHR is approximately 35-45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ghr growth hormone receptor [ Rattus norvegicus ]
• Official Symbol: Ghr
• Synonyms: GHR; growth hormone receptor; GH receptor; somatotropin receptor; growth hormone receptor/binding protein; GHR/BP; MGC124963; MGC156665
• Gene ID: 25235
• mRNA Refseq: NM_017094
• Protein Refseq: NP_058790