Recombinant Rat Hspa5 Protein, His-tagged

• Cat.No.: Hspa5-1639R
• Product Overview: Recombinant Rat Hspa5 Protein (Gln260-Glu469) with N-His tag was expressed in E. coli.

Specification

• Description: Enables misfolded protein binding activity and unfolded protein binding activity. Involved in several processes, including cellular response to cAMP; cellular response to gamma radiation; and cellular response to metal ion. Acts upstream of or within response to endoplasmic reticulum stress. Located in membrane; mitochondrion; and smooth endoplasmic reticulum. Biomarker of fatty liver disease; post-traumatic stress disorder; transient cerebral ischemia; ureteral obstruction; and visual epilepsy. Human ortholog(s) of this gene implicated in dopamine beta-hydroxylase deficiency. Orthologous to human HSPA5 (heat shock protein family A (Hsp70) member 5).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 24.6 kDa
  Accurate Molecular Mass: 25 kDa
• Protein length: Gln260-Glu469
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Hspa5 heat shock protein 5 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Hspa5
• Synonyms: HSPA5; heat shock protein 5; 78 kDa glucose-regulated protein; GRP 78; GRP-78; heat shock 70kD protein 5; heat shock 70 kDa protein 5; steroidogenesis-activator polypeptide; immunoglobulin heavy chain-binding protein; heat shock 70kDa protein 5 (glucose-regulated protein); BIP
• Gene ID: 25617
• mRNA Refseq: NM_013083
• Protein Refseq: NP_037215
• UniProt ID: P06761