Active Recombinant Rat Icoslg protein, His-tagged, Low Endotoxin

• Cat.No.: Icoslg-290R
• Product Overview: Recombinant Rat Icoslg protein(F1LVL2)(25-261 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 25-261 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat B7-H2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 28.52 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Icoslg inducible T-cell co-stimulator ligand [ Rattus norvegicus ]
• Official Symbol: Icoslg
• Synonyms: ICOSLG; inducible T-cell co-stimulator ligand; Icosl; RGD1562791
• Gene ID: 499415