Recombinant Rat Il18bp protein, His-tagged

• Cat.No.: Il18bp-1578R
• Product Overview: Recombinant Rat Il18bp aa. (Thr29~Pro193 (Accession # Q9JLN2)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Antagonist for the proinflammatory responses induced by endogenous IL-18.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.4kDa
• Protein length: Thr29~Pro193 (Accession # Q9JLN2)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.
• Isoelectric Point: 6.8

Gene Information

• Gene Name: Il18bp interleukin 18 binding protein [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Il18bp
• Synonyms: Il18bp; interleukin 18 binding protein; Igifbp; interleukin-18-binding protein; interferon gamma inducing factor binding protein
• Gene ID: 84388
• mRNA Refseq: NM_053374.2
• Protein Refseq: NP_445826.2
• UniProt ID: Q9JLN2

Reviews

06/03/2022

Consistent binding affinity.

03/01/2020

High purity.

05/04/2018

Effective in neutralization assays.

Q&As

Can you elaborate on the experimental strategies used to assess the genetic polymorphisms and variations in IL18BP that might influence its function and disease susceptibility?

Genetic association studies, sequencing analyses, and functional investigations explore how genetic variations in IL18BP might influence disease susceptibility and outcomes.

How are animal models and in vivo imaging techniques used to understand the role of IL18BP in modulating IL18-mediated immune responses in whole organisms?

Knockout mice, transgenic models, and imaging techniques provide insights into IL18BP's impact on IL18-related immune responses in vivo.

What techniques are used to investigate the regulation of IL18BP expression by cytokines, growth factors, and other signaling molecules in different cell types?

Promoter studies, siRNA knockdown, and cytokine stimulation assays investigate IL18BP regulation at the transcriptional and post-transcriptional levels.

How are cell-based assays and functional studies employed to dissect the mechanisms through which IL18BP modulates IL18-induced immune responses?

Luciferase reporter assays, ELISAs, and functional cell assays probe how IL18BP modulates IL18-induced cytokine production and immune cell activation.

What experimental approaches are utilized to explore the potential allosteric effects of IL18BP on IL18 receptor complex formation and downstream signaling?

Biophysical techniques, site-directed mutagenesis, and receptor binding assays reveal potential allosteric effects on IL18 signaling.

How do researchers experimentally characterize the binding affinity and kinetics between IL18BP and IL18, considering their critical role in regulating IL18-mediated inflammation?

Surface plasmon resonance, isothermal titration calorimetry, and kinetic binding assays quantify IL18BP-IL18 interactions.

Can you explain the strategies used to assess the expression levels and tissue distribution of IL18BP in various physiological and pathological contexts?

RT-qPCR, immunohistochemistry, and tissue microarray analyses determine the expression patterns of IL18BP in different tissues and diseases.

Could you elaborate on the methods used to investigate the structural determinants of IL18BP's interaction with IL18 and its impact on receptor binding?

X-ray crystallography, NMR spectroscopy, and mutagenesis studies elucidate the structural basis of IL18BP-IL18 recognition.

How do researchers experimentally study the potential cross-talk between IL18BP and other cytokine regulatory systems, such as IL1Ra and soluble IL6 receptor?

Cross-competition assays, co-stimulation studies, and receptor activation assays unveil potential interplay between IL18BP and other cytokine regulators.

What assays and methodologies are employed to evaluate the therapeutic potential of enhancing or inhibiting IL18BP activity in inflammatory diseases?

Therapeutic antibody trials, in vitro functional screens, and disease model assessments evaluate IL18BP modulation as a potential anti-inflammatory strategy.