Recombinant Mouse IL18BP cell lysate
| Cat.No. : | IL18BP-2674MCL |
| Product Overview : | Mouse IL18BP derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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| Source : | Human cells |
| Species : | Mouse |
| Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
| Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
| Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
| Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
| Gene Name : | Il18bp interleukin 18 binding protein [ Mus musculus ] |
| Official Symbol : | IL18BP |
| Synonyms : | IL18BP; interleukin 18 binding protein; interleukin-18-binding protein; interferon gamma inducing factor binding protein; interferon gamma-inducing factor-binding protein; MC54L; Igifbp; IL-18BP; |
| Gene ID : | 16068 |
| mRNA Refseq : | NM_010531 |
| Protein Refseq : | NP_034661 |
| Function : | interleukin-18 binding; |
Products Types
| ◆ Recombinant Protein | ||
| IL18BP-315H | Recombinant Human IL18BP Protein, His-tagged | +Inquiry |
| Il18bp-142M | Recombinant Mouse Il18bp Protein, His (Fc)-Avi-tagged | +Inquiry |
| IL18BP-252I | Recombinant Human IL18BP Protein | +Inquiry |
| IL18BP-316H | Recombinant Human IL18BP Protein, Fc-tagged | +Inquiry |
| IL18BP-194H | Recombinant Human IL18BP Protein, His (Fc)-Avi-tagged | +Inquiry |
| ◆ Lysates | ||
| IL18BP-2918HCL | Recombinant Human IL18BP cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionGenetic association studies, sequencing analyses, and functional investigations explore how genetic variations in IL18BP might influence disease susceptibility and outcomes.
Knockout mice, transgenic models, and imaging techniques provide insights into IL18BP's impact on IL18-related immune responses in vivo.
Promoter studies, siRNA knockdown, and cytokine stimulation assays investigate IL18BP regulation at the transcriptional and post-transcriptional levels.
Luciferase reporter assays, ELISAs, and functional cell assays probe how IL18BP modulates IL18-induced cytokine production and immune cell activation.
Biophysical techniques, site-directed mutagenesis, and receptor binding assays reveal potential allosteric effects on IL18 signaling.
Surface plasmon resonance, isothermal titration calorimetry, and kinetic binding assays quantify IL18BP-IL18 interactions.
RT-qPCR, immunohistochemistry, and tissue microarray analyses determine the expression patterns of IL18BP in different tissues and diseases.
X-ray crystallography, NMR spectroscopy, and mutagenesis studies elucidate the structural basis of IL18BP-IL18 recognition.
Cross-competition assays, co-stimulation studies, and receptor activation assays unveil potential interplay between IL18BP and other cytokine regulators.
Therapeutic antibody trials, in vitro functional screens, and disease model assessments evaluate IL18BP modulation as a potential anti-inflammatory strategy.
Customer Reviews (3)
Write a reviewConsistent binding affinity. -
High purity. -
Effective in neutralization assays. -
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