Recombinant Mouse IL18BP cell lysate

• Cat.No.: IL18BP-2674MCL
• Product Overview: Mouse IL18BP derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Il18bp interleukin 18 binding protein [ Mus musculus ]
• Official Symbol: IL18BP
• Synonyms: IL18BP; interleukin 18 binding protein; interleukin-18-binding protein; interferon gamma inducing factor binding protein; interferon gamma-inducing factor-binding protein; MC54L; Igifbp; IL-18BP
• Gene ID: 16068
• mRNA Refseq: NM_010531
• Protein Refseq: NP_034661
• Function: interleukin-18 binding

Reviews

06/03/2022

Consistent binding affinity.

03/01/2020

High purity.

05/04/2018

Effective in neutralization assays.

Q&As

Can you elaborate on the experimental strategies used to assess the genetic polymorphisms and variations in IL18BP that might influence its function and disease susceptibility?

Genetic association studies, sequencing analyses, and functional investigations explore how genetic variations in IL18BP might influence disease susceptibility and outcomes.

How are animal models and in vivo imaging techniques used to understand the role of IL18BP in modulating IL18-mediated immune responses in whole organisms?

Knockout mice, transgenic models, and imaging techniques provide insights into IL18BP's impact on IL18-related immune responses in vivo.

What techniques are used to investigate the regulation of IL18BP expression by cytokines, growth factors, and other signaling molecules in different cell types?

Promoter studies, siRNA knockdown, and cytokine stimulation assays investigate IL18BP regulation at the transcriptional and post-transcriptional levels.

How are cell-based assays and functional studies employed to dissect the mechanisms through which IL18BP modulates IL18-induced immune responses?

Luciferase reporter assays, ELISAs, and functional cell assays probe how IL18BP modulates IL18-induced cytokine production and immune cell activation.

What experimental approaches are utilized to explore the potential allosteric effects of IL18BP on IL18 receptor complex formation and downstream signaling?

Biophysical techniques, site-directed mutagenesis, and receptor binding assays reveal potential allosteric effects on IL18 signaling.

How do researchers experimentally characterize the binding affinity and kinetics between IL18BP and IL18, considering their critical role in regulating IL18-mediated inflammation?

Surface plasmon resonance, isothermal titration calorimetry, and kinetic binding assays quantify IL18BP-IL18 interactions.

Can you explain the strategies used to assess the expression levels and tissue distribution of IL18BP in various physiological and pathological contexts?

RT-qPCR, immunohistochemistry, and tissue microarray analyses determine the expression patterns of IL18BP in different tissues and diseases.

Could you elaborate on the methods used to investigate the structural determinants of IL18BP's interaction with IL18 and its impact on receptor binding?

X-ray crystallography, NMR spectroscopy, and mutagenesis studies elucidate the structural basis of IL18BP-IL18 recognition.

How do researchers experimentally study the potential cross-talk between IL18BP and other cytokine regulatory systems, such as IL1Ra and soluble IL6 receptor?

Cross-competition assays, co-stimulation studies, and receptor activation assays unveil potential interplay between IL18BP and other cytokine regulators.

What assays and methodologies are employed to evaluate the therapeutic potential of enhancing or inhibiting IL18BP activity in inflammatory diseases?

Therapeutic antibody trials, in vitro functional screens, and disease model assessments evaluate IL18BP modulation as a potential anti-inflammatory strategy.