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Active Recombinant Rat IL3 Protein, His-tagged

Cat.No. : IL3-1093R
Product Overview : Recombinant rat IL3 (NP_113701.1) (Met 1-Cys 169) was fused with a polyhistidine tag at the C-terminus.
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Source : Baculovirus-Insect Cells
Species : Rat
Tag : His
Predicted N Terminal : Ile 27
Form : Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% gly1, 5%~8% trehalose and mannitol.
Bio-activity : Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 2-8 ng/mL.
Molecular Mass : The secreted recombinant rat IL3 comprises 153 amino acids with and has a predicted molecular mass of 17.5 kDa. It migrates as an approximately 24 kDa band in reduced SDS-PAGE.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : Il3 interleukin 3 [ Rattus norvegicus ]
Official Symbol : IL3
Synonyms : IL3; interleukin 3; interleukin-3; IL-3; MCGF; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor;
Gene ID : 24495
mRNA Refseq : NM_031513
Protein Refseq : NP_113701
MIM :
UniProt ID :
Pathway : Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserve
Function : cytokine activity; cytokine activity; cytokine activity; cytokine activity; growth factor activity; growth factor activity; interleukin-3 receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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