Active Recombinant Rat Il4 protein, His-tagged

• Cat.No.: IL4-2702R
• Product Overview: Recombinant Rat Il4 protein(Cys25-Ser147), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 25-147 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.65-2.6 μg/mL, corresponding to a specific activity of 3.85×10^2~1.54×10^3 units/mg.
• Molecular Mass: 14.60 kDa
• Endotoxin: < 0.01 EU/μg of the protein by LAL method
• Purity: ≥ 90% as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Il4 interleukin 4 [ Rattus norvegicus ]
• Official Symbol: Il4
• Synonyms: IL4; interleukin 4; interleukin-4; IL-4; BSF-1; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IGG differentiation factor; Il4e12
• Gene ID: 287287
• mRNA Refseq: NM_201270
• Protein Refseq: NP_958427