Recombinant Rat Interleukin 18

• Cat.No.: Il18-522R
• Product Overview: Recombinant rat IL-18 is a single,non-glycosylated polypeptide containing 158 amino acids and having amolecular weight of 18.3 kDa.

Specification

• Cat. No.: Il18-522R
• Description: Interleukin-18also known as IL18 is a protein which in humans is encoded by the IL18 gene.The protein encoded by this gene is a proinflammatory cytokine.
• Biological Activity: The Rat IL-18 isfully biologically active when compared to standard. The ED50 asdetermined by the dose-dependent stimulation of IFN- gamma by murine lymphnode cells, stimulated with sub optimal dose of ConA (0.75 µg/ml) is 12ng/ml.
• Molecular Weight: 18.3 kDa
• Source: E.coli
• Species: Rat
• Form: Lyophilized (from 1mg/ml in 0.4X PBS)
• Appearance: Lyophilized protein
• Endotoxin Level: <0.1 ng/µg
• Reconstitution: Reconstitute inwater to a concentration of 0.1-1.0 µg/µl. Gentle pipetting to mix it well.The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
• Purity: >95% by SDS-PAGEand HPLC analysis
• Storage: Thelyophilized IL-18 is best-stored desiccated below 0°C. Reconstituted IL-18飥?should be stored at working aliquots at -20°C.
• Pathways: African trypanosomiasis;Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway;Hypertrophy Model; Influenza A; Legionellosis; Malaria; NOD-like receptorsignaling pathway; Rheumatoid arthritis; Salmonella infection; Tuberculosis
• Tag: Non

Gene Information

• Gene Name: Il18 interleukin 18 [ Rattus norvegicus]
• Official Symbol: Il18
• Synonyms: Il18; interleukin 18; IL-18;IGIF; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducingfactor; interferon gamma-inducing factor; interleukin 18(interferon-gamma-inducing factor); MGC12320; IL1F4; iboctadekin; IL-1g;Interferon gamma-inducing factor; Iboctadekin; Interleukin-1 gamma
• Gene ID: 29197
• mRNA Refseq: NM_019165
• Protein Refseq: NP_062038
• UniProt ID: P97636
• Chromosome Location: 8q23
• Function: cytokine activity; interleukin-18receptor binding; protein binding

Reviews

10/15/2022

Effective in inflammation models.

08/22/2021

High stability in serum.

05/09/2019

Suitable for ELISA.

Q&As

How do researchers experimentally investigate the crosstalk between IL18 and other immune signaling pathways, such as Toll-like receptor signaling?

Co-immunoprecipitation, co-localization studies, and pathway inhibition assays decipher IL18's interactions with signaling molecules and pathways.

What assays and methodologies are employed to evaluate the potential therapeutic interventions targeting IL18 in autoimmune diseases and chronic inflammation?

Therapeutic antibody trials, in vitro drug screens, and disease model assessments assess IL18-targeted interventions in autoimmune diseases.

How are animal models and in vivo imaging techniques used to understand the kinetics of IL18-mediated immune responses and its tissue-specific effects?

Xenograft models, intravital imaging, and tissue-specific knockout approaches unravel IL18's kinetics and effects within complex in vivo environments.

Can you elaborate on the techniques used to assess the impact of IL18 on immune cell activation, migration, and effector functions?

Flow cytometry, chemotaxis assays, and functional cell assays examine IL18's impact on immune cell activation, migration, and effector functions.

Can you explain the experimental strategies used to analyze the interactions between IL18 and its receptor complex, as well as the downstream signaling events triggered by receptor engagement?

Surface plasmon resonance, mutational studies, and phosphoproteomics illuminate IL18's receptor interactions and downstream signal transduction pathways.

How are cellular and molecular techniques employed to investigate IL18's role in regulating the balance between Th1 and Th2 immune responses?

Immunophenotyping, cytokine profiling, and gene expression analyses elucidate IL18's influence on Th cell polarization and immune balance.

What experimental approaches are utilized to study the genetic and epigenetic regulation of IL18 expression in response to different stimuli?

ChIP assays, promoter analyses, and epigenetic profiling uncover the regulatory mechanisms underlying IL18 gene expression dynamics.

What methods are used to dissect the mechanisms through which IL18 contributes to the pathogenesis of inflammatory diseases and its interactions with other proinflammatory molecules?

Disease models, knockout mice, and co-stimulation studies explore IL18's involvement in inflammatory conditions and its interplay with other proinflammatory mediators.

How do researchers investigate the role of IL18 in modulating the tumor microenvironment and its potential implications for cancer immunotherapy?

Tumor models, immune profiling, and immunotherapeutic studies investigate IL18's modulation of the tumor microenvironment and its implications for cancer therapy.

Could you explain the experimental strategies used to characterize the inflammasome activation triggered by IL18 and its downstream effects on cytokine production?

Caspase assays, cytokine ELISAs, and microscopy techniques unveil the activation of inflammasomes and IL18's contribution to pyroptosis and cytokine storms.