Recombinant Rat Interleukin 18

• Cat.No.: Il18-522R
• Product Overview: Recombinant rat IL-18 is a single,non-glycosylated polypeptide containing 158 amino acids and having amolecular weight of 18.3 kDa.

Specification

• Cat. No.: Il18-522R
• Description: Interleukin-18also known as IL18 is a protein which in humans is encoded by the IL18 gene.The protein encoded by this gene is a proinflammatory cytokine.
• Biological Activity: The Rat IL-18 isfully biologically active when compared to standard. The ED50 asdetermined by the dose-dependent stimulation of IFN- gamma by murine lymphnode cells, stimulated with sub optimal dose of ConA (0.75 µg/ml) is 12ng/ml.
• Molecular Weight: 18.3 kDa
• Source: E.coli
• Species: Rat
• Form: Lyophilized (from 1mg/ml in 0.4X PBS)
• Appearance: Lyophilized protein
• Endotoxin Level: <0.1 ng/µg
• Reconstitution: Reconstitute inwater to a concentration of 0.1-1.0 µg/µl. Gentle pipetting to mix it well.The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
• Purity: >95% by SDS-PAGEand HPLC analysis
• Storage: Thelyophilized IL-18 is best-stored desiccated below 0°C. Reconstituted IL-18飥?should be stored at working aliquots at -20°C.
• Pathways: African trypanosomiasis;Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway;Hypertrophy Model; Influenza A; Legionellosis; Malaria; NOD-like receptorsignaling pathway; Rheumatoid arthritis; Salmonella infection; Tuberculosis

Gene Information

• Gene Name: Il18 interleukin 18 [ Rattus norvegicus]
• Official Symbol: Il18
• Synonyms: Il18; interleukin 18; IL-18;IGIF; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducingfactor; interferon gamma-inducing factor; interleukin 18(interferon-gamma-inducing factor); MGC12320; IL1F4; iboctadekin; IL-1g;Interferon gamma-inducing factor; Iboctadekin; Interleukin-1 gamma
• Gene ID: 29197
• mRNA Refseq: NM_019165
• Protein Refseq: NP_062038
• UniProt ID: P97636
• Chromosome Location: 8q23
• Function: cytokine activity; interleukin-18receptor binding; protein binding