Active Recombinant Rat Lgals7 protein, His-tagged
Cat.No. : | Lgals7-427R |
Product Overview : | Active Recombinant Rat Lgals7 protein(P97590)(1~128 aa), fused with N-terminal His tag, was expressed in E.coli. |
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Species : | Rat |
Source : | E.coli |
Tag : | His |
Protein Length : | 1-128 aa |
Form : | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Bio-activity : | The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re-epithelialization of wounds It can agglutinate red blood.In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Ra GAL7 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL7 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL7, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown. It was obvious that the minimal effective concentration of GAL7 is 25 μg/mL. |
Molecular Mass : | 15.6kDa |
Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
Purity : | > 95% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | Lgals7 lectin, galactoside-binding, soluble, 7 [ Rattus norvegicus ] |
Official Symbol | Lgals7 |
Synonyms | LGALS7; lectin, galactoside-binding, soluble, 7; galectin-7; gal-7; lectin, galactose binding, soluble 7; |
Gene ID | 29518 |
mRNA Refseq | NM_022582 |
Protein Refseq | NP_072104 |
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Not For Human Consumption!
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