Active Recombinant Rat LIFR protein, His-tagged

• Cat.No.: LIFR-3408R
• Product Overview: Recombinant Rat LIFR protein(G3V7K2)(Met1-Ser829), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse LIF hFc at 2 μg/ml (100 μl/well) can bind Rat LIFR His, the EC50 of Rat LIFR His is 300-700 ng/mL.2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 8-40 ng/mL in the presence of 2 ng/mL recombinant human LIF.
• Molecular Mass: The recombinant rat LIFR comprises 797 amino acids and predicts a molecular mass of 90 kDa. The apparent molecular mass of the recombinant protein is approximately 116 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Length: Met1-Ser829
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lifr leukemia inhibitory factor receptor alpha [ Rattus norvegicus ]
• Official Symbol: LIFR
• Synonyms: LIFR; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF-R; LIF receptor; leukemia inhibitor factor receptor alpha-chain
• Gene ID: 81680
• mRNA Refseq: NM_031048
• Protein Refseq: NP_112310