| Species : |
Rat |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
34-202 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells . The biological effectsof TNF-β are very similar to TNF-α,due to the similarity of molecular structure and the receptors.As reported, TNF-α could inhibit the proliferation and induceapoptosis of A549 cells,therefore, A549 cells were seeded into triplicatewells of 96-well plates at a density of 4,000 cells/well with 5% serum standard DMEM including various concentrations of recombinant rat TNF-β. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37°C. Proliferation of A549 cells after incubation with TNF-β for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant mouse TNF-βfor 48h. It was obvious that TNF-β significantly inhibit cell viability of A549 cells. The ED50 is 2.5μg/ml. |
| Molecular Mass : |
22.4kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
