| Description : |
Heparin-binding growth factor; involved in early fetal development of the rat adrenal. |
| Source : |
E.coli |
| Species : |
Rat |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Bio-activity : |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml. |
| Molecular Mass : |
Approximately 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids. |
| Protein length : |
120 |
| AA Sequence : |
VAKKKDKVKKGSECSEWTWGPCTPS SKDCGMGFREGTCGAQTQRIHCKVP CNWKKEFGADCKYKFESWGACDGST GTKARQGTLKKARYNAQCQETIRVT KPCTSKTKSKAKAKKGKGKD |
| Endotoxin : |
Less than 0.1 EU/μg of rRtMidkine as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20centigrade. Further dilutions should be made in appropriate buffered solutions. |
