Recombinant Rat Pdgfrb protein(Met1-Lys530), His-tagged

• Cat.No.: Pdgfrb-8786R
• Product Overview: Recombinant Rat PDGFRB (Q05030) (Met1-Lys530) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-His
• Protein length: Met1-Lys530
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rat PDGFRB-His at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB, The EC50 of Cynomolgus PDGFB is 3-7 ng/ml.
• Molecular Mass: The recombinant rat PDGFRB comprises 510 amino acids and predicts a molecular mass of 57.6 kDa. The apparent molecular mass of the recombinant protein is approximately 92 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pdgfrb platelet derived growth factor receptor, beta polypeptide [ Rattus norvegicus ]
• Official Symbol: Pdgfrb
• Synonyms: PDGFRB; platelet derived growth factor receptor, beta polypeptide; platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta platelet-derived growth factor receptor; Platelet-derived growth factor receptor, beta; beta-type platelet-derived growth factor receptor; platelet-derived growth factor receptor beta variant 1; PDGFR-1
• Gene ID: 24629
• mRNA Refseq: NM_031525
• Protein Refseq: NP_113713