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Recombinant Rat Rab7a protein, His-tagged

Cat.No. : Rab7a-1129R
Product Overview : Recombinant Rat Rab7a protein(NP_076440.1)(Met1-Cys207), fused with His tag, was expressed in E. coli.
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  • Gene Information
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Source : E. coli
Species : Rat
Tag : His
Form : Lyophilized from sterile PBS, 10 % glycerol.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant rat Rab7a consists 225 amino acids and predicts a molecular mass of 25.7 kDa.
Protein Length : Met1-Cys207
Purity : > 90 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Rab7a RAB7A, member RAS oncogene family [ Rattus norvegicus ]
Official Symbol : Rab7a
Synonyms : RAB7A; RAB7A, member RAS oncogene family; ras-related protein Rab-7a; ras-related protein p23; ras-related protein BRL-RAS; RAB7, member RAS oncogene family; Rab7;
Gene ID : 29448
mRNA Refseq : NM_023950
Protein Refseq : NP_076440

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What signaling pathways regulate the activity of RAB7A? 11/17/2022

RAB7A activity is regulated by several signaling pathways. One important pathway involves the phosphorylation of RAB7A by protein kinases, such as LRRK2 and CDK1, which modulate its GTPase activity and subcellular localization. Additionally, RAB7A can be regulated by post-translational modifications, including ubiquitination and acetylation. These modifications can affect its interaction with effector proteins and its ability to regulate vesicle trafficking and autophagy.

What are the challenges in targeting RAB7A for therapeutic purposes? 10/31/2021

Targeting RAB7A for therapeutic purposes faces several challenges. One challenge is the need for selective modulation of RAB7A activity without affecting other closely related RAB GTPases. Additionally, the development of efficient delivery systems to specifically target RAB7A in affected cells or tissues is essential. Furthermore, understanding the precise spatiotemporal regulation of RAB7A and its interactions with effector proteins is crucial for designing effective therapeutic interventions. Overcoming these challenges will be instrumental in harnessing the therapeutic potential of targeting RAB7A in neurodegenerative diseases.

What are the potential therapeutic strategies targeting RAB7A? 09/17/2020

argeting RAB7A could be a promising therapeutic strategy for neurodegenerative diseases. One approach is to modulate RAB7A activity using small molecules or gene therapy to restore its normal function in vesicle trafficking and autophagy. Another strategy is to identify compounds that enhance the clearance of aggregated proteins through the autophagy-lysosome pathway, where RAB7A plays a crucial role. Further research is needed to develop and optimize these therapeutic interventions.

How does RAB7A interact with its effector proteins? 01/01/2020

RAB7A interacts with various effector proteins to exert its functions in intracellular trafficking. For example, it binds to RILP (RAB7A-interacting lysosomal protein) to recruit dynein-dynactin motor complexes, facilitating the movement of late endosomes towards microtubule minus ends. RAB7A also interacts with FYCO1 and PLEKHM1, which are involved in autophagosomal maturation and fusion with lysosomes. These interactions enable RAB7A to coordinate and regulate vesicle trafficking processes.

How is RAB7A involved in neurodegenerative diseases? 09/11/2019

RAB7A dysfunction has been implicated in various neurodegenerative diseases. For example, mutations in the RAB7A gene have been associated with Charcot-Marie-Tooth disease type 2B and amyotrophic lateral sclerosis. In these diseases, impaired RAB7A function disrupts the transport of essential cargoes, such as neurotrophic factors and lysosomal enzymes, leading to neuronal degeneration and dysfunction. Understanding the precise mechanisms by which RAB7A contributes to these diseases is an active area of research.

What is the role of RAB7A in intracellular trafficking? 09/21/2017

RAB7A is a small GTPase protein that plays a crucial role in regulating late endocytic trafficking. It controls the maturation of late endosomes to lysosomes by mediating the fusion of these compartments. Additionally, RAB7A is involved in autophagy, a process that degrades damaged organelles and protein aggregates. It promotes the formation of autophagosomes and their fusion with lysosomes. RAB7A also regulates the transport of cargo along the endocytic pathway.

How is RAB7A involved in the regulation of lysosomal biogenesis? 11/26/2016

RAB7A is involved in the regulation of lysosomal biogenesis. It promotes the transport of lysosomal enzymes from the trans-Golgi network to late endosomes/lysosomes. RAB7A also regulates the fusion of late endosomes with lysosomes, allowing the delivery of newly synthesized lysosomal proteins. Moreover, RAB7A interacts with transcription factors, such as TFEB and MITF, to regulate the expression of lysosomal genes, thereby influencing lysosomal biogenesis and function.

Customer Reviews (3)

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Reviews
02/15/2022

    After using this protein reagent, my protein gel electrophoresis results are notably clear, aiding in analysis and interpretation.

    06/03/2021

      Using this reagent, my experimental results are more consistent and reliable.

      02/17/2017

        Combining the instructions provided with this protein reagent, I am able to easily accomplish experimental design and execution.

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