Recombinant Rat SCGB1A1 Protein

• Cat.No.: SCGB1A1-5252R
• Product Overview: Recombinant Rat SCGB1A1 full length or partial length protein was expressed.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Scgb1a1 secretoglobin, family 1A, member 1 (uteroglobin) [ Rattus norvegicus ]
• Official Symbol: SCGB1A1
• Gene ID: 25575
• mRNA Refseq: NM_013051.1
• Protein Refseq: NP_037183.1
• UniProt ID: P17559

Citation

Lung Secretoglobin Scgb1a1 Influences Alveolar Macrophage-Mediated Inflammation and Immunity

Journal: Frontiers in Immunology    PubMed ID: 33117399    Data: 2020/10/1

Authors: Min Xu, Wei Yang, Deepak Kumar Nayak

Article Snippet:The effect of SCGB1a1 protein on AM responsiveness to inflammatory stimuli were studied in vitro .The effect of SCGB1a1 protein on AM responsiveness to inflammatory stimuli were studied in vitro .. Flow sorted AMs were plated ( ) in 12-well plates at 1 × 10 5 cells/well in triplicates and were incubated with Toll-like receptor (TLR) agonists (Invivogen) 2- heat-killed Listeria monocytogenes (HKLM), TLR4-Lipopolysaccharide from Escherichia coli K12 (LPS) and TLR5- Salmonella typhimurium Flagellin (FLA) in presence or absence of recombinant SCGB1a1 protein at 5 μg/mL (Creative BioMart).. Culture supernatant was collected and total RNA was isolated at 72 h post-stimulation.Culture supernatant was collected and total RNA was isolated at 72 h post-stimulation.

Influence of Secretoglobin family 1A member 1 ( Scgb1a1 ) deficiency on alveolar macrophage (AM) phenotypes. (A) Flow cytometric analysis of mouse AMs (CD45 + , CD11c hi , and Siglec-F hi cells) from bronchoalveolar lavage fluid. (B) AMs from WT ( Scgb1a1 +/+ ) and KO ( Scgb1a1 –/ – ) mice were analyzed for phenotypic differences at 4, 8, 12, and 40 weeks of age.

Gene expression in AMs from Scgb1a1 KO mice across age groups. (A) Up-regulated and down-regulated genes in AMs at 4, 8, 12, and 40 weeks; (B–D) Volcano plots of significantly up-regulated (red) and down-regulated (blue) genes (FDR < 0.05); (E) Venn diagrams of the significantly changed genes. Venn diagrams of the significantly changed gene sets in AMs from KO mice at different ages including GO-Molecular function pathways (F) , GO-Biological process pathways (G) , KEGG-metabolism pathways (H) , and KEGG-Disease pathways (I) . FDR < 0.05 was considered significant; FC, fold change.

Comparison of the AM gene expression patterns in Scgb1a1 KO and WT mice. (A) Up-regulated and down-regulated genes in AMs at 4, 8, 12, and 40 weeks; (B–E) . Volcano plots of significantly up-regulated (red) and down-regulated (blue) genes (FDR < 0.05); (F) PCA plot of the variant feature of gene expression; (G) Venn diagrams of the significantly changed genes. Venn diagrams of the significantly changed gene sets in AMs from KO vs WT mice at different ages including GO-Molecular function pathways (H) , GO-Biological process pathways (I) , KEGG-metabolism pathways (J) , and KEGG-Disease pathways (K) . FDR < 0.05 was considered significant; FC, fold change.