"TIMP1" Related Products

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Recombinant Rat TIMP1 Protein

Cat.No.: TIMP1-1072R
Product Overview: Recombinant rat TIMP1 (P01860) (Met1-Ala217) was expressed and purified.
Source: HEK293
Species: Rat
Predicted N Terminal: Cys 24
Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.0, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGLDPA-AR-NH2. The IC50 value is approximately 4 nM.
Molecular Mass: The recombinant rat TIMP1 consists of 194 amino acids and has a predicted molecular mass of 21.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Timp1 TIMP metallopeptidase inhibitor 1 [ Rattus norvegicus ]
Official Symbol: TIMP1
Synonyms: TIMP1; TIMP metallopeptidase inhibitor 1; metalloproteinase inhibitor 1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinases 1; Timp; TIMP-1;
Gene ID: 116510
mRNA Refseq: NM_053819
Protein Refseq: NP_446271
MIM:
UniProt ID:
Pathway: Hemostasis, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Response to elevated platele
Function: enzyme inhibitor activity; metal ion binding; metalloendopeptidase inhibitor activity; metalloendopeptidase inhibitor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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