Recombinant Rat TNF Protein

Cat.No.: TNF-1071R
Product Overview: Recombinant mature form of rat TNFα (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus.
Source: E. coli
Species: Rat
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.01-0.02 ng/mL.
Molecular Mass: The recombinant rat TNFα consisting of 157 amino acids and has a calculated molecular mass of 17.4 kDa. It migrates as an approximately 15 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >93 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Tnf tumor necrosis factor [ Rattus norvegicus ]
Official Symbol: TNF
Synonyms: TNF; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; Tnfa; RATTNF; TNF-alpha; MGC124630;
Gene ID: 24835
mRNA Refseq: NM_012675
Protein Refseq: NP_036807
MIM:
UniProt ID:
Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosyst
Function: cytokine activity; cytokine activity; identical protein binding; identical protein binding; protease binding; protease binding; transcription regulatory region DNA binding; transcription regulatory region DNA binding; tumor necrosis factor receptor bindin

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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