| Species : |
Rat |
| Protein Length : |
499 residues |
| Description : |
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms. |
| Purity : |
≥ 95% as judged by gel analysis |
| Unit definition : |
1 U of enzyme reduces 1 μmol DTNB per min in 0.5 mL standard DTNB assay containing 2.5 mM DTNB and 0.3 mM NADPH in TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5) |
| Applications : |
DTNB assay; Insulin-coupled Thioredoxin assay; Inhibitor screening; etc. |
| Notes : |
Centrifuge the tube briefly before opening the lid. |
| Concentration : |
1 mg/mL |
| Storage Buffer : |
TE buffer with 50% glycerol |
| Shipping : |
Shipped in cold pack by courier service. Store at -20 centigrade upon delivery. |
| References : |
1. Cheng Q, Arnér ES. (2017) Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation. J Biol Chem. 292:5476-5487.
2. Arnér ES, Holmgren A. (2001) Measurement of thioredoxin and thioredoxin reductase. Curr Protoc Toxicol. Chapter 7:Unit 7.4. |
