Recombinant Rhesus CCL5 protein

• Cat.No.: CCL5-3411R
• Product Overview: Recombinant Rhesus CCL5 protein was expressed in E.coli.

Specification

• Description: Chemokine (C-C motif) ligand 5 (CCL5) also known as RANTES is classified as a chemotactic cytokine or chemokine. It is encoded by the CCL5 gene in humans. CCL5 is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites which has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells. It also induces the proliferation and activation of certain natural-killer cells to form CHAK cells. Recombinant Rhesus Macaque CCL5 contains 68 amino acids and it shares 97% a.a. sequence identity with human CCL5.
• Source: E.coli
• Species: Rhesus
• Form: Lyophilized from a 0.2 µm filtered solution in 20mM PB, pH 6.0, 500mM NaCl.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.
• Molecular Mass: Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
• Protein length: 68
• AA Sequence: SPHASDTTPCCFAYIARPLPRAHIK EYFYTSGKCSNPAVVFVTRKNRQVC ANPEKKWVREYINSLEMS
• Endotoxin: Less than 0.1 EU/μg of rRhRANTES/CCL5 as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
• Tag: Non

Gene Information

• Gene Name: CCL5
• Official Symbol: CCL5
• Synonyms: CCL5; chemokine (C-C motif) ligand 5; D17S136E, SCYA5, small inducible cytokine A5 (RANTES); C-C motif chemokine 5; beta chemokine RANTES; MGC17164; RANTES; regulated upon activation; normally T expressed; and presumably secreted; SIS delta; SISd; small inducible cytokine subfamily A (Cys Cys); member 5; T cell specific protein p288; T cell specific RANTES protein; TCP228; eoCP; SIS-delta; beta-chemokine RANTES; small-inducible cytokine A5; T-cell specific protein p288; t cell-specific protein P228; T-cell-specific protein RANTES; eosinophil chemotactic cytokine; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; D17S136E
• Gene ID: 574178
• mRNA Refseq: NM_001032850.1
• Protein Refseq: NP_001028022.1
• UniProt ID: Q8HYQ1

Reviews

08/17/2021

This specificity enables precise quantification and characterization of CCL5 and its interactions with other molecules or proteins of interest.

06/23/2019

Its exceptional performance, coupled with its ability to generate clear and precise protein bands, make it an indispensable tool in achieving accurate and reliable results.

06/20/2016

The CCL5 protein exhibits a high degree of specificity in various assays, ensuring accurate and reliable results.

Q&As

What are the potential clinical applications of CCL5 inhibitors?

CCL5 inhibitors may be used to control inflammation in diseases like rheumatoid arthritis or as an adjuvant in cancer therapy.

Can CCL5 be used to stimulate the immune system in cancer treatment?

CCL5-based immunotherapies are being explored to enhance the immune response against cancer cells.

Can CCL5 be used as a diagnostic marker for diseases?

It can serve as a biomarker for some diseases, but it is usually used in conjunction with other markers for more accurate diagnostics.

What are the clinical implications of elevated CCL5 levels?

Elevated CCL5 levels are associated with inflammation and may be indicative of certain diseases such as rheumatoid arthritis or HIV infection.

Are there any CCL5-targeted therapies in development?

Yes, there are ongoing efforts to develop therapies that modulate CCL5 activity, particularly in diseases involving inflammation.