Recombinant Rhesus macaque LILRB1 protein, His-tagged

• Cat.No.: LILRB1-01R
• Product Overview: Recombinant Rhesus macaque LILRB1 protein(F7H3G7)(Ser17-His456), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rhesus macaque
• Tag: C-His
• Protein length: Ser17-His456
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Anti-LILRB1 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque LILRB1, His Tag with an affinity constant of 215.30 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.