Recombinant Rhesus macaque TGFB1 protein, His-tagged

• Cat.No.: TGFB1-6544R
• Product Overview: Recombinant Rhesus macaque TGFB1 protein(F7HCV5)(Leu30-Ser390(C33S)), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: Leu30-Ser390(C33S)
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rhesus macaque Latent TGF beta 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 0.74ug/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.4/12.8 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.