Recombinant S. cerevisiae ULP1 protein

• Cat.No.: ULP1-1571S
• Product Overview: Recombinant protein Saccharomyces cerevisiae (yeast) Ulp1 was expressed in E. coli.

Specification

• Species: S.cerevisiae
• Source: E.coli
• Tag: Non
• Description: Yeast Ulp1 is a highly active protease which specifically recognizes the tertiary structure of ubiquitin-like protein, SUMO rather than a short sequence as other
  commonly used proteases such as Thrombin, TEV, and EK. The protease can be used to cleave the SUMO from recombinant fusion proteins at 30 centigrade or 4 centigrade at pH 7.0-7.9. After digestion, the His-Tagged Ulp1 can be easily removed by His tag affinity chromatography.
• Form: 50 mM Tris-HCl, (pH7.5), 150 mM NaCl, 1 mM DTT, and 50% glycerol.
• Molecular Mass: 27 kDa
• Unit Definition: 1U cleaves 85% of 100 ug of sumo-fusion protein at 30 centigrade for 1 hour or 80% at 4 centigrade overnight in a buffer containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, and 10% glycerol.
• Storage: Stable for 2 years at -70 centigrade from date of shipment. Please aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: ULP1 SUMO protease ULP1 [ Saccharomyces cerevisiae S288c ]
• Official Symbol: ULP1
• Synonyms: ULP1; SUMO protease ULP1
• Gene ID: 856087
• mRNA Refseq: NM_001183834.1
• Protein Refseq: NP_015305.1
• UniProt ID: Q02724