Recombinant Saccharomyces Cerevisiae KAE1 Protein (1-386 aa), His-tagged

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Recombinant Saccharomyces Cerevisiae KAE1 Protein (1-386 aa), His-tagged

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Cat.No. : KAE1-1750S
Product Overview : Recombinant Saccharomyces Cerevisiae (strain ATCC 204508/S288c) (Baker's yeast) KAE1 Protein (1-386 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length.
Description : Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. KAE1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.
Source : Yeast
Species : Saccharomyces Cerevisiae
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 44.7 kDa
Protein length : 1-386 aa
AA Sequence : MVNLNTIPPK NGRDYYIALG LEGSANKLGV GIVKHPLLPK HANSDLSYDC EAEMLSNIRD TYVTPPGEGF LPRDTARHHR NWCIRLIKQA LAEADIKSPT LDIDVICFTK GPGMGAPLHS VVIAARTCSL LWDVPLVGVN HCIGHIEMGR EITKAQNPVV LYVSGGNTQV IAYSEKRYRI FGETLDIAIG NCLDRFARTL KIPNEPSPGY NIEQLAKKAP HKENLVELPY TVKGMDLSMS GILASIDLLA KDLFKGNKKN KILFDKTTGE QKVTVEDLCY SLQENLFAML VEITERAMAH VNSNQVLIVG GVGCNVRLQE MMAQMCKDRA NGQVHATDNR FCIDNGVMIA QAGLLEYRMG GIVKDFSETV VTQKFRTDEV YAAWRD
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Synonyms : KAE1; Kinase-associated endopeptidase 1 N6-L-threonylcarbamoyladenine synthase Short name: t(6)A synthase t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 tRNA threonylcarbamoyladenosine biosynthesis protein KAE1;
UniProt ID : P36132
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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