Recombinant Streptomyces avidinii SA Protein, His-tagged

• Cat.No.: SA-06S
• Product Overview: Recombinant Streptavidin (aa 25-183) from Streptomyces avidinii was expressed in E. coli using N-terminal His tag.

Specification

• Species: Streptomyces Avidinii
• Source: E.coli
• Tag: His
• Protein Length: 25-183
• Description: Streptavidin (SA) is a tetrameric protein originating from the bacterium Streptomyces avidinii. Each subunit of the streptavidin tetramer can associate with one biotin small vitamin molecule. Although the SA-biotin interaction is non-covalent, it has an extremely high affinity (Kd ~10-14 M) . The SA-Biotin complex is highly thermostable and resistant to extreme pH, denaturing reagents, and enzyme degradation. By targeting biotin to biomolecules this interaction with SA can be exploited in a number of biochemical assays.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Storage Buffer: 50mM Na2HPO4, pH7.5, 300mM NaCl, 200mM imidazole, 1mM DTT, 10% glycerol.

Gene Information

• Official Symbol: SA
• Synonyms: SA; streptavidin