Recombinant Streptomyces coelicolor A3(2) AROB protein, His-tagged

• Cat.No.: AROB-1359S
• Product Overview: Recombiant Streptomyces coelicolor A3(2) AROB full length or partial length protein was expressed.

Specification

• Source: E. coli or Yeast
• Species: Streptomyces coelicolor A3(2)
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 80% by SDS-PAGE
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage buffer: PBS buffer

Gene Information

• Gene Name: aroB 3-dehydroquinate synthase [ Streptomyces coelicolor A3(2)]
• Official Symbol: AROB
• Synonyms: AROB; 3-dehydroquinate synthase; SC9C5.18c
• Gene ID: 1096920
• Protein Refseq: NP_625774
• UniProt ID: Q9KXQ6

Reviews

03/27/2023

Its high purity and stability ensure reliable and reproducible results, which are paramount for achieving meaningful scientific outcomes.

12/21/2020

The AROB protein is renowned for its exceptional quality and reliability, making it an ideal choice to fulfill my experimental requirements.

08/24/2020

With the AROB protein's high-quality composition and reliability, researchers can confidently proceed with their studies, knowing that they have access to a protein that consistently delivers accurate results.

08/11/2020

This commitment to staying at the forefront of research translates directly to the quality and performance of the AROB protein in my trials.

07/25/2019

the AROB Protein's applicability in protein electron microscopy structure analysis is remarkable.

04/21/2017

This protein has consistently demonstrated outstanding results, making it an indispensable tool in my research endeavors.

Q&As

Are there any known mutations in the AROB gene that can affect protein function?

Yes, mutations in the AROB gene have been identified in certain organisms. These mutations can alter the structure or activity of the AROB protein, leading to functional changes in the shikimate pathway. Such mutations can affect the efficiency of the catalytic reaction or the protein's stability, potentially impacting the biosynthesis of aromatic amino acids and other downstream compounds.

Is there any variation in the AROB gene sequence among different species?

Yes, there can be variation in the AROB gene sequence among different species. As organisms evolve, their genetic sequences can accumulate changes, resulting in genetic diversity. This genetic diversity is often reflected in variations in the AROB gene sequence. These variations can contribute to differences in the efficiency or function of the AROB protein among species.

What are the implications of targeting the AROB protein for drug development?

Targeting the AROB protein could have potential applications in drug development. Inhibitors of AROB could be explored as antimicrobial agents to disrupt the shikimate pathway in bacteria or parasites. By inhibiting the synthesis of aromatic amino acids, these inhibitors could potentially impair the growth and survival of pathogens while minimizing the impact on human cells, as humans do not possess the shikimate pathway. However, further research and understanding are required to ensure the effectiveness and selectivity of such inhibitors.

Is the AROB protein localized to a specific cellular compartment?

Yes, the AROB protein is often found in the cytoplasm of cells where the shikimate pathway takes place. Within the cytoplasm, it interacts with other enzymes and substrates involved in the pathway to carry out its catalytic function.

Can the AROB protein have any applications in biotechnology?

The AROB protein and enzymes involved in the shikimate pathway have been studied for their potential applications in biotechnology. One area of interest is the biosynthesis of aromatic compounds, which are widely used in industries such as pharmaceuticals, flavors, and fragrances. By understanding and manipulating the AROB protein and the shikimate pathway, researchers aim to engineer microorganisms or plants that can produce desired aromatic compounds in a more sustainable and cost-effective manner. Additionally, the shikimate pathway and AROB protein have been investigated for their role in the production of biofuels from renewable feedstocks.

How is the AROB protein related to other enzymes in the shikimate pathway?

The AROB protein is part of a series of enzymes that work sequentially in the shikimate pathway. It acts on the substrate 3-dehydroquinate (DHQ) to produce 3-dehydroshikimate (DHS). The resulting DHS is then processed by another enzyme in the pathway. These sequential reactions ultimately lead to the synthesis of aromatic amino acids and other aromatic compounds.

Can the AROB protein be used as a target for herbicides?

Yes, the shikimate pathway, including the AROB protein, is targeted by some herbicides. Since plants have an active shikimate pathway, herbicides designed to inhibit enzymes in this pathway can selectively hinder their growth without affecting animals or humans that lack the pathway. By inhibiting AROB, herbicides can disrupt the synthesis of aromatic amino acids in plants, leading to their impairment or death.

Can mutations in the AROB gene lead to any human diseases or disorders?

Currently, there is no evidence to suggest that mutations in the AROB gene directly contribute to human diseases or disorders. The shikimate pathway, including the AROB protein, is not essential for humans, as we obtain aromatic amino acids from our diet. However, disruptions in the shikimate pathway can indirectly affect human health if they impact the production of essential compounds in certain bacteria or parasites that depend on this pathway. In such cases, AROB inhibitors or medications targeting the shikimate pathway may have therapeutic potential for specific bacterial or parasitic infections.

Are there any structural studies on the AROB protein?

Yes, structural studies have been conducted on the AROB protein in several organisms. X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy techniques have provided insights into the three-dimensional structure of AROB. These studies have revealed valuable information about the active site, molecular interactions, and conformational changes associated with its catalytic function.

Is the AROB gene conserved across different organisms?

The AROB gene is generally well-conserved across different organisms, as the shikimate pathway and the associated enzymes are essential for the synthesis of aromatic compounds. However, there can be variations and differences in the AROB gene sequence among species. These variations can reflect adaptations to different environmental conditions or metabolic requirements. Despite these variations, the overall function and catalytic activity of the AROB protein remain largely conserved across different organisms.

Does the AROB protein have any physiological or medical significance?

The AROB protein plays a crucial role in many organisms, including bacteria, fungi, and plants, by enabling the synthesis of aromatic amino acids and other aromatic compounds. Since aromatic amino acids are essential for protein synthesis and are involved in various cellular processes, the AROB protein's correct functioning is crucial for normal growth and development. Additionally, the shikimate pathway has been explored as a potential target for the development of antimicrobial and herbicidal agents.

Are there any inhibitors or drugs that target the AROB protein?

While there are no known specific inhibitors or drugs that directly target the AROB protein, there have been studies on the development of molecules that inhibit enzymes in the shikimate pathway, including AROB. These inhibitors can potentially disrupt the biosynthesis of aromatic compounds in bacteria, fungi, or parasites, leading to therapeutic effects against specific infections. However, further research is needed to fully explore the potential of AROB inhibitors as antimicrobial agents.

Are there any known inhibitors or activators of the AROB protein?

Various inhibitors and activators of the AROB protein have been identified. For example, some compounds can competitively inhibit the binding of DHQ to AROB, impairing its catalytic activity. On the other hand, specific factors or molecules can enhance the activity of AROB, promoting the conversion of DHQ to DHS.