Recombinant Swine IL-12 p40

• Cat.No.: IL12B-84S
• Product Overview: Swine IL-12/IL-23 p40 was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.

Specification

• Description: IL-12 is a member of the IL-12 family, which includes IL-12, IL-23, IL-27, and IL-35. Like other IL-12 family members, IL-12 is a heterodimeric cytokine. IL-12 is encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer, and a homodimer of p40 are formed following protein synthesis. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-gamma and TNF-alpha from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-gamma.
• Source: Yeast
• Species: Swine
• Form: Lyophilized
• Molecular Mass: 34.3 kDa
• AA Sequence: IWELEKNVYV VELDWYPNAP GEMVVLTCNT PEEDGITWTS DQSSEVLGTG KTLTIHVKEF GDAGQYTCRK GGAVLSQSLL LLHKKEDGIW STDILKDQKE PKNKSFLKCE AKNYSGRFTC WWLTAISTDL KFSVKSSRGS TDPRGVTCGT ATLSEDLGEY KKYRVECQEG SACPAAEESL PIEVVLEAVH KLKYENYTSS FFIRDIIKPD PPKNLQLNPL KNSRHVEISW EYPDTWSTPH SYFSLMFGVQ VQGKNKREKK DKLFTDQISA KVTCHKDANI RVQARDRYYS SSWSEWASVS CN (302)
• Applications: The Swine IL-12/IL-23 p40 protein can be used in cell culture, as an IL-12/IL-23 p40 ELISA Standard, and as a Western Blot Control.
• Storage: -20 C
• Tag: Non

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.