Active Recombinant Feline IL12B Protein, His-tagged

• Cat.No.: IL12B-902F
• Product Overview: Recombinant Feline IL12B (Accession # O02744) Ile23-Ser329, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Feline
• Tag: His
• Predicted N Terminal: Ile23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to compete with biotinylated feline IL-12/IL-23 p40 for binding with immobilized rhIL-12 R beta 1/Fc Chimera in a functional ELISA assay.
• Molecular Mass: Recombinant Feline IL12B, His-tagged has a calculated MW of 36 kDa. In SDS-PAGE migrates as 42 kDa and 45 kDa, reducing conditions.
• Protein length: Ile23-Ser329
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IL12B interleukin 12B [ Felis catus (domestic cat) ]
• Official Symbol: IL12B
• Synonyms: IL-12.p40; interleukin-12 subunit beta; interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL-12B; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin 12 p40 subunit; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; interleukin-12 p40 chain
• Gene ID: 768273
• mRNA Refseq: NM_001077413.1
• Protein Refseq: NP_001070881.1
• UniProt ID: O02744
• Chromosome Location: chromosome: A1