Active Recombinant Feline IL12B Protein, His-tagged
|Product Overview :||Recombinant Feline IL12B (Accession # O02744) Ile23-Ser329, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.|
- Gene Information
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|Source :||Mammalian cells|
|Predicted N Terminal :||Ile23|
|Form :||Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.|
|Bio-activity :||Measured by its ability to compete with biotinylated feline IL-12/IL-23 p40 for binding with immobilized rhIL-12 R beta 1/Fc Chimera in a functional ELISA assay.|
|Molecular Mass :||Recombinant Feline IL12B, His-tagged has a calculated MW of 36 kDa. In SDS-PAGE migrates as 42 kDa and 45 kDa, reducing conditions.|
|Protein length :||Ile23-Ser329|
|Purity :||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Storage :||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).|
|Gene Name :||IL12B interleukin 12B [ Felis catus (domestic cat) ]|
|Official Symbol :||IL12B|
|Synonyms :||IL-12.p40; interleukin-12 subunit beta; interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL-12B; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin 12 p40 subunit; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; interleukin-12 p40 chain|
|Gene ID :||768273|
|mRNA Refseq :||NM_001077413.1|
|Protein Refseq :||NP_001070881.1|
|UniProt ID :||O02744|
|Chromosome Location :||chromosome: A1|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
ChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
Customer Reviews (3)Write a review
Good for cytokine assays. -
Effective in Th2 cell differentiation. -
High yield in expression. -
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