Recombinant Tags
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Cat.No. : | Tag-002 |
Product Overview : | Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. |
Description : | Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. |
Source : | E. coli |
Form : | Lyophilized |
Molecular Mass : | ~40 kDa |
AA Sequence : | It contains 16 commonly used tags from N terminus to C terminus in the order as shown in the table below. Trx: MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA T7: CMASMTGGQQMG HSV: QPELAPEDPEDC c-Myc: EQKLISEEDLC VSV-g: YTDIEMNRLGK Glu-Glu: CEEEEYMPME V5: GKPIPNPLLGLDST E-tag: GAPVPYPDPLEPR Flag-tag: DYKDDDDK S-tag: KETAAAKFERQHMDS HA: YPYDVPDYAC KT3: KPPTPPPEPET E2: GVSSTSSDFRDR AU1: DTYRYI AU5: TDFYLK 6x His: HHHHHH |
Storage : | Store the protein at -20 centigrade or below. After reconstitution, aliquot and store at -20°C or below. |
Concentration : | 200 µg of purified Multiple Tag (lyophilized) |
Reconstitution : | Add 200 µL of ddH2O to make the final concentration of 1 µg/µL in PBS Buffer. 0.04-0.2 µL of Multiple Tag is recommended for each lane of the minigel (for accurate loading, dilute with PBS Buffer). |
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