| Cat.No. : |
Tag-002 |
| Product Overview : |
Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. |
| Description : |
Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. |
| Source : |
E. coli |
| Form : |
Lyophilized |
| Molecular Mass : |
~40 kDa |
| AA Sequence : |
It contains 16 commonly used tags from N terminus to C terminus in the order as shown in the table below.
Trx: MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
T7: CMASMTGGQQMG
HSV: QPELAPEDPEDC
c-Myc: EQKLISEEDLC
VSV-g: YTDIEMNRLGK
Glu-Glu: CEEEEYMPME
V5: GKPIPNPLLGLDST
E-tag: GAPVPYPDPLEPR
Flag-tag: DYKDDDDK
S-tag: KETAAAKFERQHMDS
HA: YPYDVPDYAC
KT3: KPPTPPPEPET
E2: GVSSTSSDFRDR
AU1: DTYRYI
AU5: TDFYLK
6x His: HHHHHH |
| Storage : |
Store the protein at -20 centigrade or below. After reconstitution, aliquot and store at -20°C or below. |
| Concentration : |
200 µg of purified Multiple Tag (lyophilized) |
| Reconstitution : |
Add 200 µL of ddH2O to make the final concentration of 1 µg/µL in PBS Buffer. 0.04-0.2 µL of Multiple Tag is recommended for each lane of the minigel (for accurate loading, dilute with PBS Buffer). |
