Recombinant Toxoplasma Gondii Profilin Family Protein

• Cat.No.: TGME49_093690-2378T
• Product Overview: Recombinant Toxoplasma gondii full length profilin (aa 1-163) produced inE. coliis fused to a N-terminal tag. 47kDa.

Specification

• Cat. No.: TGME49_093690-2378T
• Description: The initial recognition of pathogens during host defense is mediated by a family of receptors termed toll-like receptors (TLR). TLRs recognize conserved microbial components, termed pathogen-associated molecular patterns (PAMPs). To date, 11 TLRs have been cloned in mammals and each receptor appears to be involved in the recognition of a unique set of PAMPs that are distinct in their chemical nature and structure. TLR11 has a role in uropathogenic E. coli infection and senses the profilin protein from the protozoan parasite Toxoplasma gondii. Profilins are a class of small actin-binding proteins present only in eukaryotic cells. T. gondii profilin shares significant homology only with profilin genes from other apicomplexan protozoa, such as the malaria parasite, Plasmodium falciparum, suggesting that TLR11 may be involved in the recognition of these parasites.
• Source/Host: E. coli.
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Concentration: 0.1mg/ml after reconstitution.
• Reconstitution: Reconstitute with 100µl sterile, endotoxin-free water. Further dilutions should be made with medium containing 5% fetal calf serum.
• Specificity: Binds to mouse TLR11.
• Biological Activity: Activates and induces TLR11-dependent IL-12p40 production from dendritic cells.
• Purity: ≥95% (SDS-PAGE).
• Specific Activity: 50-100ng are required for standard HAT assay.
• Formulation: Lyophilized. Contains PBS.
• Long Term Storage: -20°C.
• Use/Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Handling: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: TGME49_093690 profilin family protein [ Toxoplasma gondii ME49 ]
• Synonyms: TGME49_093690; profilin family protein
• Gene ID: 7900190
• mRNA Refseq: XM_002370130
• Protein Refseq: XP_002370171